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The intensity of a probe and thus also its -coordinates depend on the concentration of RNA-transcripts and on the binding constants for specific and non-specific hybridization as well (see Eq. (5)).
Particularly, one gets the mean PM/MM-ratios of the binding constants for specific and non-specific hybridization (24) s c ≡ K c PM, S K c MM, S ≈ 10 α c + Δ c (0 ) and n c ≡ K c PM, N K c MM, N ≈ 10 Δ c (0 ), respectively, and the mean binding strength for non-specific binding of the particular chip, (25) log X c PM, N ≈ − β c + 1 2 log n c, in analogy with Eqs.
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However, the more efficient amplification step in sample B results in a higher total number of full length transcripts and/or in a larger binding constant for non-specific binding and thus in an increased binding strength of non-specific binding which, in turn, gives rise to the increased level of cross-hybridization as indicated by the slightly narrower hook-curve.
The stepwise and overall binding constants for Ca2+ binding to NCaBD are expressed as follows.
In this model, the determined binding affinity constants become intrinsic binding constants for the individual EF-hands.
Equilibrium binding constants for the axial coordination of inorganic anions were determined by cyclic voltammetry measurements.
The equilibrium binding constants for DB244 are 10-20 times greater than that for DB226.
A majority of the binding constants for arsenic protein binding were obtained indirectly.
(E ) Summary of kinetic and thermodynamic binding constants for each class of binding event.
Isothermal titration calorimetry (ITC) was performed to determine binding constants for the binding of hemin to apo-LmCld.
Surface plasmon resonance was applied to determine the exact binding constants for HO-3.
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