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The division of these classes arises from a problem in their derivation and results in the need to use two different binding constants for one binding event.
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This result is perhaps due to the altered ability of GKRP-P446L to bind glucose besides fructose phosphates (Supplementary Figure S7), which would result in increased, but separate, binding constants for the complex (one for GK and the other for GKRP).
However, our ITC data clearly indicates that the macroscopic and microscopic binding constants for NCaBD, assuming an independent model, are one-order higher than the reported values, and also the binding for one site is one order stronger than the other (Table 2).
The stepwise and overall binding constants for Ca2+ binding to NCaBD are expressed as follows.
In this model, the determined binding affinity constants become intrinsic binding constants for the individual EF-hands.
Equilibrium binding constants for the axial coordination of inorganic anions were determined by cyclic voltammetry measurements.
The equilibrium binding constants for DB244 are 10-20 times greater than that for DB226.
Comparison of the binding constants for the binding of Mg+2 and Mn+2 to UDP-Glc to the binding of Mg+2 and Mn+2 to UDP affords an approximately 10-fold decrease in binding.
A strong NADH binding in contrast to weaker NAD+ binding of the protein was inferred from fluorometrically determined binding constants for the dinucleotide cofactor.
A majority of the binding constants for arsenic protein binding were obtained indirectly.
(E ) Summary of kinetic and thermodynamic binding constants for each class of binding event.
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