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To address this possibility, we compared two calcium indicators, 100 μM Fluo-4 (kd = 345 nM) to 100 μM Fluo-5F (kd = 2.3 µM), which have approximately sevenfold different binding constants for calcium.
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The stepwise and overall binding constants for Ca2+ binding to NCaBD are expressed as follows.
In this model, the determined binding affinity constants become intrinsic binding constants for the individual EF-hands.
Equilibrium binding constants for the axial coordination of inorganic anions were determined by cyclic voltammetry measurements.
A majority of the binding constants for arsenic protein binding were obtained indirectly.
(E ) Summary of kinetic and thermodynamic binding constants for each class of binding event.
Isothermal titration calorimetry (ITC) was performed to determine binding constants for the binding of hemin to apo-LmCld.
Surface plasmon resonance was applied to determine the exact binding constants for HO-3.
FP is a technique that can be used to obtain binding constants for macromolecular interactions.
These findings correlated well with available thermodynamic measurements of binding constants for the ligands studied.
But for determination of binding constant for bio-5'-AMP, 5 µM BPL was used.
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