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Recombinant WT and I164E-mutated Abl SH2 domains displayed indistinguishable low micromolar binding constants for an optimal Abl SH2-domain phoshopeptide ligand.
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To ensure that ATPγS recapitulates interactions formed between RIG-I and ATP, we also determined RNA binding constants for Walker A mutants in the presence of ATP.
However, our ITC data clearly indicates that the macroscopic and microscopic binding constants for NCaBD, assuming an independent model, are one-order higher than the reported values, and also the binding for one site is one order stronger than the other (Table 2).
The binding constants for the association of a cAMP molecule with the PKA tetramer are the same for all cAMP bound states of PKA, as well as the dissociation constants.
However, placing PEG at the end of the 3′- dT n tail increases the binding affinities to their maximum values (i.e. the same as binding constants for RecBC or RecBCD to a DNA end with only a 3′- dT n tail).
(36) This mutant provided useful insights into the possible role of protein flexibility in the catalytic process, but it was noted that its binding constants for NADPH and FH2 are lowered by a factor of 10.
A majority of the binding constants for arsenic protein binding were obtained indirectly.
The variability in conditional binding constants for Pb2+ and Zn2+ adsorption (log KADS) derived from a simple non-electrostatic surface complexation model were also predicted by extracted Fe and Al normalized to surface area.
Isothermal titration calorimetry experiments were used to acquire binding constants for the inhibitor MP265 and nucleotides that bind to the active site of ΔCcMreBdh.
Evaluation of binding constants for the fullerene/1 systems evoke that 1 may be employed as an efficient molecular tweezers for C70 in toluene as selectivity in binding is determined to be ∼9.4.
FP is a technique that can be used to obtain binding constants for macromolecular interactions.
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