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As a result, no binding constants could be determined from the NMR titrations.
Interestingly, the same binding constants could be determined when either the ligand or the receptors were immobilized.
Measurements were performed in the presence of 200 mM phosphate so that the binding constants could be determined from the ITC measurements (51, 61).
Similar(57)
Although no crystals could be obtained with NbGAK_2, this Nb showed the highest affinity to GAK target in comparison with all other Nbs tested in direct binding experiments using SPR (Table 1), whereas no rate and equilibrium-binding constants could be determined in a direct binding assay for the fourth Nb due to unspecific binding (results not shown).
Similar work studied human serum albumin (HSA) for plasma protein drug binding [ 24], where equilibrium constants could be estimated for several drugs tested.
This comes in contrast to all previously considered test cases (as they do not include any competing binding reactions), where the delayed reactions rate constants could be fixed to an arbitrarily high value, or where using modification M3 resulted in enhanced accuracy.
Little to no fluorescence quenching was observed when P27, P31, P35*, and P6 were titrated with the unmodified hASLLys3UUU; thus, binding constants could not be extracted from the data (Table 4).
This may be compared to a 60-fold increase demonstrated for 1. Attempts to fit these small spectral changes to binding models failed to produce reliable saturation binding curves and thus a DNA binding constant could not be derived for 2 using this latter technique.
A characteristic binding motif could be deduced.
However, no binding media could be identified in our studies.
This binding could be inhibited by heparin.
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