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In this paper, we report on the study of interaction between Chl and BSA in aqueous solution by using UV-Vis spectroscopy, which allowed the determining of the binding constants at different temperatures.
Binding constants at different temperatures obtained by using the Benesi-Hildebrand equation were found to be of the same order of magnitude (~10 M−1) indicating low affinity of Chl with BSA.
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Ka is the binding constant at different temperature.
The binding constants (K) at different temperatures and thermodynamic parameters, enthalpy changes (ΔH) and entropy changes (ΔS) were calculated according to the fluorescence data.
The W67,68 mutant was used to determine binding constants at the nonannular sites on KcsA.
Previous reports have shown that these binding constants are different in some cases [46].
We use c to denote constants and may denote different constants at different occurrences.
We did not mean to imply that studying association constants at different concentrations is not useful.
Although the absolute value of binding constants decreased drastically, differences in the binding efficiencies amongst the nucleotides became obvious at higher surface pressures.
On the contrary, T4 *-binding to TTL, besides being very low, was constant at different T4 concentrations, showing a non-specific binding.
An assay can also have one or more components, which are separate data fields that naturally fall together into an assay (e.g., the binding constant to a receptor at different concentrations).
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