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Therefore, the temperature dependence of the binding constant was studied.
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The binding constant was calculated to be 4.57 × 104 M−104
The binding constant was determined as described previously [ 29].
The binding constant is the product of a charge-independent quantity, the intrinsic binding constant, and a cooperativity function.
Consequently, the binding characteristics of paeoniflorin with HSA including the quenching mechanism, quenching and binding constants were investigated in this study, by using fluorescence quenching method through the thermodynamic analysis.
The absorption and emission properties of the ligands L1 L5 were studied in CH3CN; their coordination properties towards phosphate, pyrophosphate, and hydroxide anions, together with binding constants were evaluated.
The binding constants were measured from two sets of experiments.
The binding constants were calculated by Stern Volmer method.
The quenching constants and binding constants were determined for pSCX4 FL and pSCX4-FL Cu2+ systems.
The number of binding sites and binding constants were calculated using a double logarithm regression equation.
The binding constants were calculated using Origin provided by ITC200.
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