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The apparent binding constant of each variant was obtained using six analyte concentrations (2 – 20 nM).
The binding constant of each interaction is expressed as 1/ KA= KD (in mol·l−1) for more clarity.
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In order to complete the ITC results, surface plasmon resonance (SPR) experiments were carried out to determine the binding constants of each αRep for the EGFP target.
If in a considerable simplification of the system, we consider the RNA/SNF/U2A′ complex in terms of a two-state SNF equilibrium ensemble, α is given by (see the Supporting Information) 6where KC is the equilibrium constant between the two states of SNF and β and γ are the ratios of the binding constants of each state of SNF for each ligand.
At each temperature, binding constant of MB dimers to agar in section 2B is less than that of MB monomers to agar in section 2A of adsorption isotherm, Table 1.
The optical studies indicated that TMPyP binds to the TTAA region of d GCTTAAGC 2 with a binding constant of 2.5×106 M−1 and a stoichiometric ratio of 1 1.
All test BZTs potently bind at Sudlow site I of HSA with a binding constant of 104 L/mol at 298 K.
Kinetic studies showed that the peptide VGGVVIA binds to PLA2 in a competitive manner with a binding constant of 5.2 x 10-7 M.
The binding constant of the Zn2+ azofluorescein complex was determined to be 2.4 × 104 M−104
The binding constant of DNSCH towards Fe3+ was determined and found to be 1.5 ± 0.4 × 10−4 M−10−4
GA and BSA form a 1 1 complex with a binding constant of 9.12×105 L mol−1.
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