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Our system is label-free and scalable and enables analysis of different aptamer-target pairs and binding conditions with the same platform.
Our system is label-free and scalable and enables analysis of different aptamer target pairs and binding conditions with the same platform.
The same binding conditions with the 4 n Dome3 and 4 n Dome4 sites also give clear band shifts that can be supershifted (and possibly stabilized) by anti-GFP (Fig 3B).
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The hydrogel was shown to bind Cu2+ and Cd2+ strongly under non-competitive binding conditions, with binding capacities of 5.3 and 5.1 μmol cm−2, respectively.
The binding capacity of each metal decreased, under competitive binding conditions (with a range of metal ions present at 17.8 μN), to 1.3 and 0.17 μmol cm−2, respectively, indicating stronger selectivity for Cu2+.
Finally, in experiments at high concentrations leading to essentially stoichiometric binding, conditions with greater than 2-fold excess of EGFP over mAb led to a complex s value of 7.55 S, whereas with 2.1-fold excess of mAb over EGFP, an average complex s value of 7.18 S was observed (data not shown).
The axial flow XT5 capsule and the radial flow XT140 capsule from Pall are rigorously analyzed under binding and non-binding conditions with bovine serum albumin (BSA) as test molecule.
With the selection of optimal binding conditions a complete release of the template could be achieved upon UV irradiation, albeit with a lower binding specificity.
Because the antiporter is only minimally active at pH4 and pH8 (see Figure 4), we are unable to measure the binding affinity under these conditions with the methods available to us.
With the static binding conditions as presented here, one would also identify low-affinity receptors.
We have repeated the Monte-Carlo simulations with different parameters, binding conditions and number of spheres, and a more detailed description of these simulations can be found in the Supplementary data.
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CEO of Professional Science Editing for Scientists @ prosciediting.com