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If we had spelled out binding conditions for new entry, the discussion would not have strayed well beyond Poland, the Czech Republic and Hungary.Of the criteria you mention, I am most concerned about the question of border disputes.
Cells were grown in low binding conditions for 14 days and collected for analysis.
Different binding conditions for prothrombin exposed different epitopes, resulting in the detection of various subgroups of aPT.
Then, the upper aqueous RNA-rich phase was isolated and ethanol absolute (1.5 volumes) was added to provide appropriate binding conditions for all RNA molecules, including miRNAs.
Affinity differences may explain a reduced binding and neutralization of heterologous virus in vivo, as the binding conditions for (VN) antibodies are likely more stringent in vivo than in the in vitro SN assays.
ZRM (Francis et al., 2011, 2012) has been developed for quantitatively analyzing the impact of radially inhomogeneous flow distributions on measured chromatograms under non-binding and binding conditions for axial flow configurations.
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In titrating increasing amount of P19 in the presence of an excess of siRNA, we first established optimal binding condition of wild type P19 for siRNA.
The 5′ RNA-biotin-streptavidin beads were incubated with plastid extracts prepared from leaves of the C4 plant amaranth, using preparatory and binding conditions previously optimized for these leaves [ 26].
For strong binding conditions, confocal laser scanning microscopy (CLSM) shows asymmetrical intraparticle concentrations profiles and enhanced rates of IgG adsorption on POROS HS 20 at 1000 cm/h.
The differences between in vivo and in vitro binding conditions will likely be larger for those BH3-only members with a transmembrane domain, resulting in dissociation constants values measured in vitro that are significantly different in vivo.
The AUC studies utilized binding conditions identical to those used for the EMS experiments, except that the duplexes were labeled with 6-carboxyfluorescein (6-FAM).
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