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Half-maximal binding concentrations were obtained from a 4-parameter binding equation fit to the data.
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Final protein concentrations were obtained by Coomassie dye binding assay.
Estimated concentrations were obtained from equations (3a) and (3b) for data obtained from a 60 MHz relaxometer.
Serum IL-6 concentrations were obtained.
The protein concentration was obtained by a dye-binding assay and immunoblot was used to evaluate the binding of the panel of mAbs produced.
The concentration was obtained using a standard curve prepared from known concentrations of Lov solution.
Warfarin partition coefficients (Kps) and binding parameters were obtained from the literature (Table 3 ); absorption rate constants (ka s) and clearance parameters were obtained by fitting the PBPK model to previously reported plasma concentration time profiles.
IC50 values (peptide concentration that gives 50% binding) were obtained by nonlinear least-squares fitting of the data to a four parameter sigmoidal equation (XLfit3, IDBS).
The binding mechanism, binding constants, binding sites and binding distance were obtained.
GATA1 binding peaks were obtained from [ 15].
The values of Kd for D2-receptor binding were obtained from the literature, where Emax models were used to fit D2RO and plasma drug concentration data obtained from position emission tomography studies.
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