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Evidence supporting the function of the binding component in riboflavin uptake by the intact cells includes the following.
Here we aimed to identify the unknown chromatin binding component(s) of the A-tether.
Membrane-associated binding activity for riboflavin has also been demonstrated in membrane vesicles prepared from B. subtilis, and the binding component can be "solubilized" with Triton X-100.
Total binding was plotted vs. the concentration of [89Zr]Zr-DFO-trastuzumab; the data were fit via non-linear regression with a one-site binding model in GraphPad Prism 7.00 to determine Bmax, KD and the non-specific binding component.
The Escherichia coli phnD gene is hypothesized to code for the periplasmic binding component of a phosphonate uptake system.
This result indicates NS6740-mediated blofkade of a specific binding component.
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The inhibitor (CF-2) thus appears to be an ATP-binding component that regulates proteolysis within the 1500-kDa complex.
Kawasaki, I. et al. PGL-1, a predicted RNA-binding component of germ granules, is essential for fertility in C. elegans.
SWI4 encodes the DNA-binding component of the SBF transcription complex, which has a major role in regulating several G1-specific genes, including DNA synthesis genes27.
Similarly the Ki determined for the inhibitory analogue 5-deazariboflavin in the uptake assay and the KD for its interaction with the riboflavin-binding component of membrane vesicles are in the same range.
The Ca2+-dependent regulator protein is also structurally similar to troponin-C, the Ca2+-binding component of muscle troponin and Ca2+ mediator of muscle contraction.
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