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Thus, methyl green, a DNA major groove binder [25], [26] was used as a major groove binding competitor for this competitive experiment.
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We next used LDI/MALDI to monitor the dissociation of host guest complexes using ADA, a strong binding competitor for CB[7].
As for the assumed binding competitor (GPO)10, K d values were determined using standard conditions for SPR measurements (Table 2).
Therefore, our study indicates that this direct protein ligand binding competitor assay can be used as an effective alternative method in the early screening of PPARγ ligands.
A WT oligonucleotide was used as a binding competitor.
The aim of this study was to characterize non-substrate-binding structures for gelatinase in the ECM and the potential of synthetic collagen-like binding competitors to modulate MMP availability or activity through exosite interaction in fibrotic diseases.
We would be direct competitors for this job.
This study was designed to test the PPARγ binding potency of several major FRs, including FM550 and PBDEs along with their metabolites using a ligand-binding competitor assay.
As a result, we infer that Ir-LBP, by binding LTB4, is a competitor for the host BLT1 and BLT2 membrane receptors.
When the competition ELISA was repeated using equimolar concentrations of Fn and DNA to compete with the GST-ComE1, DNA was again a much better competitor for binding to GST-ComE1 (Fig. 1b).
Several JAK2 kinase domain inhibitors, that are ATP-binding competitors are in clinical trials for primary or secondary myelofibrosis [42], [43].
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