These truncated forms lack DNA-binding domains, but retain T3-binding competence [ 39].
Upon addiction of micromolar amounts of Zn2+, the protein undergoes a refolding to the native form and reacquires DNA-binding competence [ 56].Trying to investigate the cause of this alteration we found that the exposure to nanomolar concentrations of the beta-amyloid peptide 1-40 (Aβ 1-40) induced the expression of the unfolded p53 protein isoform in fibroblasts derived from non-AD subjects [ 3].
In any case, the differential impact of the R100 mutation on the Akt and PLC−γ1, TrkA signaling streams, as well as their reduced p75NTR binding and signaling competence, add new insights into the more general issue of NGF and pain [5], [53].
Laurenceau et al. suggest that type IV pilus-like structures directly mediate DNA uptake in pneumococci; Balaban et al. propose that DNA uptake is independent of DNA binding to the competence pilus.
The dissection of SNAP-25 binding interactions and catalytic competence have marshalled a 66-amino acid (141 206) fragment to prominence.
The role of ATP-dependent transport and DNA binding domains in pneumococcal competence [ 26], histidine kinase in sensing environmental cues and virulence [ 27], and the role of PsaA in virulence [ 18, 28], are well documented.
Uncertainty about the structure of competences also undermines international comparisons and the development of binding international agreements for consistent competence standards.
This demonstrates the competence of the binding and translocation domains of PE to mediate delivery of the DTA and RTA catalytic domains into cells cytoplasm and, importantly, that NS3 cleavage mediated enhancement in the enzymatic activity of the two zymoxins (as was detected by in-vitro assays) is practically translated to enhancement in cytotoxic activity.
It is not surprising because the DNA-binding ability and the transactivation competence of HSF1 are regulated independently.
This combination of DNA- and protein-influenced positioning regulates DNA accessibility and competence for regulatory protein binding and transcription.
