Sentence examples for binding column from inspiring English sources

In brief, 4× lysis buffer was used to break phytoplankton cells and flow through the DNA binding column, eluted by elution buffer in the end.

After the addition of 100µl isopropanol, the lysate was transferred to the upper reservoir of the binding column tube and centrifuged at 8000 rpm for 1 minute.

The gDNA extraction method (CTAB method combined with DNA binding column purification step) has been proved to be effective in removing potential PCR inhibitors rich in algal species [11].

Total cellular Rho, or activated Rho which was isolated by incubating the cell extract with an activated Rho affinity binding column (GST-tagged Rho-binding domain of Rhotekin), were detected by western blot with mouse anti-Rho A,B,C mAbs provided by the Rho Activation Assay Kit according to the manufacturer's manual (Upstate).

To each sample was added 100 µl chloroform, mixed, centrifuged at 4°C, and the aqueous supernatant was mixed with similar volume of 70% ethanol and loaded on a SV Total RNA Isolation System (Promega) RNA binding column and processed according to directions.

A binding column will be used to capture the baits.

When virions from both cell lines were affinity-purified on HLA-DR binding columns, Gag processing was reduced in those produced in the HLA-DR-expressing cells as compared to virions from cells which express CIITA (Figure 5F, upper panel and 5G), suggesting that HLA-DR alone does not contribute to increased Gag processing.

Afterward, samples were run through DNA binding columns and spun gently.

The lysed cells were mixed thoroughly by pipetting and were transferred into RNA binding columns assembled in collection tubes.

RNA binding columns were centrifuged at 10.000 × g for 1 min and the flow-though was discarded.

Furthermore, estrogen binding columns, which do not retain compounds interacting with AhR only, enabled us to purify and concentrate ER ligands.