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Both 7-1C and 67-7A bound the ECD peptide with apparent Kd values ranging between 1.2 n M and 1.6 n M. Binding characteristics were further evaluated in flow cytometry analyses performed with cells stably expressing SAIL.
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Newborn characteristics were further categorized as male or female.
Compounds that showed promising binding characteristics were then further assessed for inhibition of HIV-1 infection using a well-characterized luciferase reporter gene neutralization assay.
The binding characteristics were analyzed by biosensor-based experiments.
In vitro αvβ3-binding characteristics were determined in a competitive binding assay.
The specific organic binding media were further analyzed by Py-GC/MS.
Binding characteristics of A38 to VACV were further validated by dot blot analysis of immobilized VACV particles stained with biotinylated A38 (OligoService, Berlin, Germany).
The binding characteristics of each anti-CAIX scFvFc were further analyzed by SPR through a capture technique, where purified anti-CAIX scFvFc was captured onto a BiaCore CM4 CHIP immobilized with anti-human IgG-Fc antibodies.
The region of p65 binding was further narrowed to identify specific binding determinants (data not shown).
Specific binding was further confirmed by immunofluoresence microscopy.
There were further items on sociodemographic and illness characteristics.
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