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However, this mutational scheme failed to induce significant binding changes in the already-high-affinity C225 Fab/huEGFR interface.
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To evaluate the pH dependent variations in binding, changes in both the ligand and in the enzyme had to be taken into account.
Leaks to German newspapers and news agencies suggest that Berlin is moving swiftly to complete proposals for a binding change in repayment terms in the event of default.
[10, 11] Even if a binding event does not significantly change the size or charge of a protein, MST can still detect the binding owing to binding- induced changes in the molecules' solvation entropy.
Fluorescence enhancements were also observed for molecules upon DNA binding, with changes in the emission which were much more dramatic for DNA binding by prodigiosin.
PAPS binding was monitored via binding-induced changes in the intrinsic fluorescence of SULT1A1.
Increasing ionic strength did not significantly influence the binding, whereas changes in the mobile phase pH led to a re-orientation on SP Sepharose FF.
Upon calcium binding, conformational changes in the CaM/M13 complex cause a fluorescence change in the circularly permuted GFP-based fluorophore [18].
Mobilization of CaM from nucleus to distal dendrites could be caused by the dissociation of CaM from its nuclear binding components, changes in the permeability of the nuclear pore complex for free diffusion, and/or transported by a carrier.
The signaling of the sensor is based on target binding-induced changes in the conformation and flexibility of the methylene blue-modified aptamer probe.
Microring resonators are label-free sensors that support spectrally well-defined optical modes that are extremely sensitive to surface binding-induced changes in the local refractive index.
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