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The open binding chamber of AcrB (PDB ID: 3AOD, chain A) was studied with four ligands.
In the HSP60 system, ATP binding brings about a conformational change that exposes its peptide binding core allowing peptides to enter the peptide binding chamber [ 28, 29].
Equally the electron transfer blockade may be due to the alteration in the tight sealing of the quinone binding chamber close to N2 after the movement of the ND3 hydrophilic loop and ND1.
Such an ATP-bound resting state does not hinder substrate access to the putative binding chamber at the TMD interface, since ATP binding does not induce closure of the cytoplasmic side of BmrCD.
It is possible that deactivation of mitochondrial complex I leads to the disruption of the enclosed quinone binding chamber due to a concerted structural rearrangement of these two crucial membrane subunits.
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FamD1 adopts a typical ABBA architecture of aromatic prenyltransferase, in which the substrate-binding chamber is found in the central β-barrel.
Because unbound actin filaments were washed out of the chamber, all binding events occurred on the surface, so this number allows us to account for all actin-bound cortactin molecules in the chamber.
Cells were plated overnight in 8-well chamber slides; binding of collagen-coated beads in the presence of pre-immune serum or β1 integrin blocking antibody was analyzed after 1 hour bead incubation.
Cameron, who has said that any deal agreed with fellow EU leaders would be legally binding, sought assurances that the chamber would wave through the legal changes – a promise that the parliament's president, Martin Schulz, withheld.
Proteins in the reaction buffer were injected into the chamber to measure binding to DNA substrate.
Similar experiments in a flow chamber model evaluated binding of spherical particles ranging from 100 nm to 10 μm coated with endothelial ligand.
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