Sentence examples for binding cells were incubated from inspiring English sources

In order to avoid nonspecific binding, cells were incubated with 0.5 µg Fc blocker (per 106 cells) for 30 minutes on ice followed by staining with rabbit anti-mouse MMP-9.

For protein binding, cells were incubated with human IgG-Fc fusion proteins or with MuHV-4 glycoprotein-specific mAbs (1h, 4°C), washed ×2 in PBS, and then incubated with fluorescein-conjugated rabbit anti-mouse IgG pAb (Dako Cytomation) or phycoerythrin-conjugated goat anti-human IgG-Fc pAb (Sigma Chemical Co).

To prevent non-specific binding, cells were incubated with FcR Blocking Reagent (Miltenyi Biotec).

To analyze immunotoxin binding, cells were incubated with HM1.24-ETA′ or control proteins at indicated concentrations for 30 min on ice.

For antibody evaluation and for visualization of toxin binding, cells were incubated with BoNT/A holotoxin and complex for 4 h.

After blocking with buffer (PBS/ anti-human Ab (1 : 500)) to eliminate nonspecific binding, cells were incubated with primary antibodies (1 : 200; OVN at 4 °C) and FITC-conjugated secondary antibodies at 37 °C for 1 h.

For homogeneous binding assay, cells were incubated in binding buffer with 200 pM of I-GLP-1 and different concentrations of unlabelled GLP-1 (21.4 pM–166.7 nM) at room temperature for 3 h.

For the binding assay, cells were incubated with increasing concentrations of Rh-labeled EGF, and binding was analyzed using flow cytometry.

To determine specific versus nonspecific binding, the cells were incubated with the unlabeled CaIX-P1 peptide at concentrations varying from 10−4 to 10−10 mol/L.

Following washing with binding buffer cells were incubated with a rabbit anti-human C4BP antibody, followed after washing with swine anti-rabbit FITC labeled antibodies (DAKO).

To characterize CPMV binding the cells were incubated with CPMV-AF488 for 3 hours on ice, washed, stained and analyzed by FACS.