We initially characterized the effects of AR ligand binding on ARIBE cells using a luciferase reporter system, and examined changes in AR response genes using qPCR.
This study was designed to examine the identity of nicotine-binding sites on immune cells using a human leukaemic monocytic cell line, THP-1, that is known to have functions that are modulated by nicotine.
Moreover, we too have purified additional putative SIRT3-binding proteins from HeLa cells (using a related cross-linking/immunoaffinity purification method [ 47]), which include mitochondrial acetoacetyl CoA thiolase (also referred to as α-ketothiolase), malate dehydrogenase, thioredoxin 2 (Trx-2), Hsp60, and lactate dehydrogenase (unpublished data).
Hybridoma culture supernatants were tested for binding to purified recombinant CD70 protein by ELISA and for binding to formalin-fixed CD70-positive cells using a FMAT8200 instrument (Applied Biosystems, Foster City, CA, USA).
Digman, M. A. & Gratton, E. Analysis of diffusion and binding in cells using the RICS approach.
In this study, we decided to solve the confusing issue of detection of the pan-caspase Z-VAD-FMK substrate binding to dying unicellular cells using Leishmania as a model system.
We next verified the interaction between USP7 and its binding proteins in cells using co-immunoprecipitation to investigate which site (TRAF or the acidic pocket on TUDUSP7) on USP7 is responsible for the interaction with distinct binding proteins.
631958) that permits flexible assay design for the rapid identification of STAT3 transcription factor DNA binding in cell extracts using an ELISA (enzyme-linked immunosorbent assay) format was used to determine the STAT3 DNA binding activity.
The levels of TSP binding to M3Da. cells evaluated by binding studies, using an anti-TSP monoclonal antibody (MAb) (LYP8), shows 367,000 +/- 58,000 (mean +/- s.d).
In order to determine the binding affinity, the variants were competitively analyzed in an enzyme-based assay on rhPSMA (NAALADase assay) and in a cell binding assay with LNCaP cells using 67Ga-labeled 6 (radiochemical purity > 99%, specific activity approximately 1,000 GBq/μmol) as radioligand.
