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We calculated the relative DC-SIGN binding capacity with the strongest DC-SIGN binding milk arbitrarily set to 100%.
When labeled, these probes enable the delineation of the status and any alterations of cellular binding capacity, with the potential to detect disease-associated changes [ 5, 20, 21].
The BDE-47 metabolite 3-OH-BDE-47 (IC50 = 0.24 μM) showed a similar binding capacity with the positive control rosiglitazone, followed by 5-OH-BDE-47 with a calculated IC50 of 3.09 μM.
As can be seen, with the DEAE Sepharose FF column the dynamic binding capacity and therefore the amount of enzyme that should be applied to the column to keep loss at a minimum, is very low (about 60 units) compared to the dynamic binding capacity with the Q Sepharose XL column (about 180 units).
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Br-Mac-12 has a more efficient binding capacity with DNA than the corresponding DTAB without the fluorophore.
We demonstrated that the tandem peptide provides an approximately twofold increased binding capacity with respect to the linear and branched peptides, that could be attributed to a lower rate of dissociation (Kd).
9, 13 The gel's binding capacity with water, which is the intrinsic characteristic of hyaluronic acid, is influenced by several already mentioned properties.
This clearly demonstrates the advantage of the micro-sized latex particles where the polymeric PSA is capable to intensify the probe binding capacity with a simple loading method via the classical EDC/NHS coupling compared to avidin-biotin technology [24, 26] and ultra-low detection limit in zeptomolar range with reasonable assay time.
L-2 showed (Fig. 3b) the maximum binding capacity with Ni2+, Pb2+ and Hg2+ and the values were 80.02, 83.63 and 68.97%, respectively, indicating higher number of ligands that interacted with metal ions.
To obtain the highest possible binding capacity with fewer photografting steps, we investigated the post-polymerization grafting via two grafting approaches, i.e. a single-step approach that includes monomer and initiator in the grafting solution and a two-step approach that allows the covalent attachment of the initiator, followed by a graft polymerization reaction from the pore surface.
In silico analysis using bioinformatics softwares of Alibaba2.0 (Niels Grabe, http://www.gene-regulation.com/pub/programs/alibaba2/) and TESS program (http://agave.humgen.upenn.edu/utess/tess), predicts that the C allele has stronger binding capacity with transcriptional factor sp1 when compared with the T allele.
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