p53 activity was measured by its DNA binding capacity using an ELISA system (TransAm ELISA; Active Motif, Carlsbad, USA).
Plasma haptoglobin concentration was determined as the haemoglobin binding capacity using an appropriate assay (TP801: Tridelta Development Ltd., Greystones, Ireland), which was previously validated [ 32] and was quantified using a spACE automated analyser (Alfa Wassermann, Inc., West Caldwell, NJ, USA) [ 33].
The avidity could be increased by generation of recombinant antibodies with multivalent binding capacity using the scFv multimerization approach or generation of a fusion with the whole Fc fragment of human IgG [ 26, 34, 35].
Both versican and CCL2 are upregulated in SSc monocytes; to determine whether these form a complex, we examined CCL2's binding capacity using plastic plates coated with or without synthetic CS.
Here, we have focused instead on defining the amino acid residues of L22 that contribute to its RNA binding capacity using two RNAs that match the binding site consensus previously defined by Dobbelstein and Shenk [25].
Therefore, to directly assess the effect of altered cell-surface uPA on plg-binding capacity, we used a model breast cancer cell line (MCF-7) that has very low constitutive cell-surface uPAR and modulated its expression by PMA stimulation [ 32].
An MCF-7 HER2 negative breast cancer cell line with low trastuzumab binding capacity was used as a negative control.
Instead, we incubated several individual resin samples under a range of NaOH concentrations simultaneously, then washed them with binding buffer prior to static binding capacity assays using stock mAb solution.
Clay swelling could be reduced significantly by transforming clay minerals into non-expandable binding materials with cementing capacity using alkaline activation.
Different techniques, including polarized light microscopy, synchrotron XRD, mechanical analyses, and oil binding capacity were used to study the physical and mechanical properties of the palm oil-oleogel systems and chocolate spreads.
