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The first investigates the effect of pH and salt concentration on the binding of green fluorescent protein, isolated from Escherichia coli homogenate, to a weak anion exchange resin and the second examines the impact of salt concentration, pH and initial feed concentration upon the binding capacities of a FAb′, isolated from E. coli lysate, to a strong cation exchange resin.
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Chloride binding isotherms reflect the chloride binding capacity of a specific cementitious material in concrete (Luping and Nilsson 1993).
Additionally, the dynamic binding capacity of a methacrylate monolith column for S. aureus phages VDX-10 was determined.
Specific binding capacity of a diol-containing dye Alizarin Red S in the gels at pH 7.0 coincided with the content of immobilized NAAPBA indicating the 1 1 stoichiometry of the reaction and, therefore, good accessibility of the boronic acid ligands for water-soluble diols.
This work explores the impact of three pore sizes (with dextran distribution coefficients of 0.4, 0.53, and 0.64), dextran surface extender concentration (11 20 mg/mL), and ligand density (77 138 μmol H+/mL resin) of cation exchange resins on the dynamic binding capacity of a therapeutic antibody.
Experimental verification of binding capacity of a random sample of additional predicted motifs is underway in our laboratory.
The structural analysis is also crucial to the binding capacity of a selectin molecule to PSGL-1 ligand under blood flow, since the conformational change of Lec domain is usually accompanied with the action of mechanical force [7].
However, the membrane binding capacity of a C-peptide with an altered heptad repeat sequence and containing a PBD but not a LBD (PBD-4HR, similar do C34) is very poor, similar to a C-peptide that does not have any of these domains [26].
The SPR results, along with data from F NMR and steady-state primer kinetics, elucidate how lesion-induced conformational heterogeneity alters the binding capacity of a polymerase and thus its nucleotide insertion efficiency.
Gel shift assays demonstrate consistent differences in the binding of Rel family proteins to DNA, since acute TNF stimulation induces formation of a dominant DNA binding complex comprising p65 p50 heterodimers (complex I), while the binding capacity of a second complex (complex II), which may include p50 p50 homodimers, is increased in nuclear extracts of chronic TNF-stimulated cells.
Based on this analysis, we created N-terminally FLAG-tagged truncated TIMELESS mutants, whose N terminus also contains a canonical SV40 nuclear localization signal, in order to avoid mischaracterization of the binding capacity of a mutant that cannot properly localize to the nucleus.
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