The PAZ (Piwi, Argonaut and Zwille) domain has nucleic acid-binding capability and is potentially involved in post-transcriptional gene silencing [ 16].
It was therefore postulated that all karyopherins must have RanGTP binding capability, and this assertion has been validated in almost all cases so far.
One interpretation of these data is that the acidic residues in the QacR multidrug binding pocket contribute primarily to polyspecific binding capability and drug orientation.
It has been previously demonstrated that FOXP3 binding capability is sensitive to cellular Ca2+ influx and that it can be dramatically increased in T cells upon stimulation with PMA and ionomycin [ 16].
Most recently, Ernst and colleagues [8] found that ligand binding capability is inherent to the PDZ domain, and mutated variants can support specificities that do not exist in nature, suggesting that this structural and functional flexibility could be exploited to facilitate rapid rewiring of protein-protein interaction networks during evolution [9].
Whether this susceptibility is due to an alternative structure or reduced binding capability to Hfq is unclear.
Weaker reduction of the DNA binding capability is observed when D242 (position −1 ZF3), D270 (position −1 ZF4), S272 (position +2 ZF4) are mutated to alanine.
Additionally, this binding could be completed by the chemically synthesized APWHLSSQYSRT peptide, indicating that the binding capability was specific and conferred by the peptide sequence.
The probe sensing conditions such as solvent effects, concentration, competitive metal/anion ions, pH, measurement time and ion binding capability were comprehensively investigated and optimized.
The polymorphisms of the fractalkine receptor, that codify for a molecule with minor expression and with a reduced binding capability, have been proposed as a novel protective marker for coronary disease.
