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Consequently, binding can be monitored by measuring the change in coupling geometry (i.e., the angle) between the metal film and the excitation source needed to generate PSPs [106, 107].
CFP-PAcKer binds more tightly to a full-length variant of PAK1 that is stabilized in the "open" state (Kd = 3.3 μM) than to full-length PAK1 in the "closed" state (undetectable affinity), and binding can be monitored with fluorescence by placing an environmentally sensitive fluorescence dye on CFP-PAcKer adjacent to the binding site.
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The binding event can be monitored both by absorption spectrometry and by fluorescence spectrometry.
The work reported herein is an initial feasibility study to show that antibody-antigen binding events can be monitored at the single molecule level using single molecule localisation microscopy (SMLM).
Furthermore, by the use of a non-coupled bead, non-specific binding (NSB) can be monitored and corrected for.
For example, by using a fusion protein of GR and FP, the dynamics of the exchange of GR with its binding sequence array can be monitored [ 77].
The radiolabeled RGD peptide accumulates in chronically inflamed tissue in mice due to of αvβ3-specific binding, and this process can be monitored noninvasively with PET [17].
Therefore, the extent of substrate binding to Ec-GluRS can be monitored by fluorescence titration experiments.
The binding of peptides to micelles can be monitored by relaxation enhancements in a paramagnetic environment.
However, the overall binding capacity of the library can be monitored precisely with the presence of fluorescence tag.
The binding of daptomycin to a GUV can be monitored by the expansion of the membrane area.
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