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When starting from the dimeric state (5 mM NH4Ac, pH 8.8), rising ATP concentration induces an increase of the probe affinity, suggesting that ATP binding can also induce a dimer-monomer transition in this same buffer.
Binding can also induce a conformational alteration to the protein and hence affect the function of the protein.
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On the other hand, studies suggest that binding of TRAIL to these receptors can also induce proliferation through activation of the NF-κB signalling pathway [ 23, 24], and it appears that the ability of TRAIL to trigger either apoptosis or cell survival depends on the cell type [ 25].
Although the transcription-dependent functions of p53 seem to be most important – 90% of cancer-related p53 mutations occur within the DNA binding domain (Soussi et al., 2005) – p53 can also induce apoptosis independently of its role as a transcription factor.
To investigate if BH2 can also induce high-affinity binding, we incubated the enzyme overnight in the presence of 25 μM BH2.
Yet another exorphin, deltorphin, binds to your delta opioid receptors, which can also induce pain relief.
In addition, E2 can also induce the production of sex hormone-binding globulin thus reducing the amount of free E2 in the blood 24, 25.
For instance, besides acting as a conventional TF, some HMG-box TFs can also induce changes in the DNA structure that enhance binding of other TFs [ 34].
TGF-β can also induce HSP47 expression by enhancing the heat shock element binding activity of heat shock transcription factor (Sasaki et al., 2002).
Calcium binding in forisomes is also weak and probably non-specific given that other divalent cations can also induce the typical conformational change [ 31, 12].
Ligation of integrins can also induce intracellular signaling and the activation of integrins to a higher-affinity binding state by 'inside-out' signaling.
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