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Cysteine mutants with altered ligand binding can also be selected simultaneously by affinity chromatography.
However, while Myc binding sites are frequently proximal to altered H3 methylation sites, peaks of Myc binding can also be found several kb away.
A useful insight into conformational rearrangements in MotB required for peptidoglycan (PG) binding can also be gained through determination of the structure of MotB-C complex with a peptidoglycan fragment.
Antibody-mediated inhibition of Wnt binding can also be detected using the smaller Wnt-binding fragments, E3-E4 for Wnt3a and E1-E2 for Wnt9b (Figure 6C and 6D).
Non-sequence-specific binding can also be driven by a locally permissive chromatin structure [ 3, 10].
Not forgetting, of course, that plasma protein binding can also be nonlinear and non-time constant.
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23 Many reviews on protein-DNA interactions are available, such as Rohs et al 24 The functionality after DNA-binding can also be influenced by the DNA structural properties as many biological processes require the DNA molecule to adapt to a specific conformation, for example a DNA-loop which can facilitate protein-protein interactions at long distances.
In the present study, we aimed to investigate whether pH-dependent binding sites can also be introduced into antigen binding IgG1-Fc [fragments of immunoglobulin G (Fcab)] molecules.
More complex regulatory systems, in which there is differential binding affinity or cooperativity among multiple binding sites, can also be applied (see supplementary material Section 4 and Fig. S3) (Parker et al., 2011).
Hereby, binding site-based classification outnumbers sequence-based classifications since similar binding sites can also be found in more distant proteins.
Transcription factor binding specificities can also be seen by comparing the Hth and Ubx binding sites.
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