Sentence examples for binding buffer with a from inspiring English sources

Next, the His-tagged TrxA protein was eluted from the column using binding buffer with a gradient of increasing imidazole concentrations (up to 500 mM imidazole).

AnnexinV and antibodies were filtered with a 0.1-µm filter (Millipore, Darmstadt, Germany), and PBS and AnnexinV-binding buffer with a 0.2-µm syringe filter (Sarstedt, Nümbrecht, Germany).

The complex was washed thrice by incubating for 10 min (with agitation) in wash buffer (binding buffer with 100 units/ml RNasin).

Complexes were washed three times with binding buffer with corresponding concentrations of locostatin or DHPE and boiled in sample buffer (2×).

After mixing for 1 hr at 4°C, the beads were collected and washed 3 times with bead binding buffer with 5 mM imidazole before analysis by immunoblotting.

The beads were then incubated in DNA binding buffer with 3 million biotinylated reverse primers per bead for 2 hours, and washed in PBS.

Briefly, cells were incubated in binding buffer with R-phycoerythrin annexin-V antibody for 5 min, followed by flow cytometric analysis.

Briefly, cells were incubated in 100 uL annexin binding buffer with 5 uL annexin V-APC antibody for 15 minutes at room temperature, washed, and analyzed.

Complexes were washed three times with binding buffer with corresponding concentrations of RKIP binding compounds (98, 26 or 48) or locostatin or DHPE and boiled in sample buffer (2X).

The columns were equilibrated with PBS binding buffer with 20 mM imidazole.

These reactions were also incubated in binding buffer with the C2-L1Tc protein (0.67 μM) at 37 °C for 5 min.