Sentence examples for binding buffer which was from inspiring English sources

Then 5 μl annexin V FITC was added to 195 μl of the cell suspension binding buffer, which was prepared by diluting the binding buffer 1 4 in distilled water (50 ml binding buffer +150 ml distilled water).

Briefly, 2 × 10 cells were co-cultured with 90 ug/ml of GST-TAT-Apoptinopt for 24 hrs, and then resuspended in 100 ul of 1× binding buffer, which was followed by incubation with 2.5 ul of Annexin-V FITC and PI at room temperature for 30 min.

Cells were resuspended into 500  μl of binding buffer, to which was added 5  μl annexin V-FITC and 5  μl of propidium iodide (PI).

After samples were mixed with binding buffer, which inhibits RNase activities, 25 fmol of synthetic cel-miR-39 was spiked.

The harvested cells were suspended in 200 μL binding buffer which contained 10 μL Annexin V-FITC and PI.

The binding of proteins to the surface of the array can also be influenced by the amount of ACN in the binding buffer where higher ACN restricts the binding to proteins which are more hydrophobic than the buffer [ 65].

The supernatant was passed through a 3-mL nickel-nitriloacetic acid (Ni-NTA) column, which was washed with binding buffer (25 mL) and binding buffer containing 25 mM imidazole (25 mL).

Finally, 400 μl of binding buffer was added to each sample, which was held on ice prior to analysis on a FACSCalibur (Becton Dickinson Labware, Franklin Lakes, NJ) flow cytometer.

Protein binding was evaluated during an association phase (0 300 sec), which was followed by a dissociation phase (injection of buffer only, 300 3700 sec).

Binding protein was eluted by the same buffer, the eluted protein was pooled and loaded on an anion-exchange Mono Q column which was equilibrated with 20 mM Tris HCl buffer (pH 8.2).

The samples were stored in the dark at room temperature for 15 min after which 400  μl of binding buffer was added and annexin V flow cytometry was performed within 1 h, analysing 10 000 cell events.