Sentence examples for binding buffer were used from inspiring English sources
Untreated cells re-suspended in DH20 for 15 minutes prior to adding binding buffer were used as a positive control for dead cells.
Ultra-15 Centrifugal Filters [30,000 MWCO; Millipore, Billerica, MA] equilibrated with binding buffer were used to reduce the volume to ~2 ml.
For FCM, cells (5 × 10 cells/ml) suspended in 500 μl binding buffer were used for DNA stain with 5 μl Annexin V-FITL and propidium iodine (PI, Sigma); then, DNA value, cell cycle and apoptotic rate of each group were determined by a cell apoptotic detection kit (BioDev, China) and a fluorescent activated cell sorter (420 type FCM, Becton-Dickinson, USA) as described previously [ 9, 12, 23].
Subsequently, binding buffer was used to wash the column, followed by elution buffer (20 mmol/L Tris HCl pH 7.9, 500 mmol/L imidazole, 0.5 mol/L NaCl).
After that, 15 ml of binding buffer was used to wash the column and then the r AciHBGase II-(His 6 protein was eluted with 5 ml of elution buffer (binding buffer except with 150 mM imidazole).
QIAquick 96 PCR purification cartridges (Qiagen, Hilden, Germany, with modified binding and wash buffers) were used to remove unincorporated primers before tags were decoupled from amplification products by UV photolysis in a flow cell and analyzed in a quadrapole mass spectrometer by using positive-mode atmospheric pressure chemical ionization (APCI-MS, Agilent Technologies, Palo Alto, CA, USA).
Blocking buffer was used to avoid non specific binding.
RIPA buffer was used to reduce non-specific binding of actin to sepharose beads.
Thus far, all fluorescence measurements were carried out in a phosphate pH 7 buffer, as this buffer was used to study fluorescein-HSA binding in the past.
Figure 2 showed the real-time SPR binding curves in the injection of sample, while the PBS buffer was used for the SPR running buffer.
