Sentence examples for binding buffer were transferred from inspiring English sources

The resuspended cells in 100 μL of 1 × binding buffer were transferred to a 1.5 mL culture tube, and 5 μL of FITC Annexin V and PI were added to each tube.

A 100 μL of the suspended cells in binding buffer was transferred to a 5-mL culture tube, and 5 μL of Annexin V-FITC and 10 μL of PI were added.

After staining, 350 μl of Annexin V binding buffer was added and all of the samples were transferred to Falcon flow tubes for analysis using FACSCanto Flow Cytometer (BD Bioscience, USA).

After incubation, the reaction mixture was made up to 100 µl with binding buffer before being transferred to a streptavidin-coated 96-well plate and incubated at 37°C for 1 h.

The cells were then washed twice with 0.5 M NaCl in binding buffer before being transferred to fresh tubes for counting using a microbeta scintillation counter (LKB Wallac; Perkin Elmer, Cambridge, UK).

400 μL of 1X binding buffer was added to stop the staining reaction.

Briefly, Protein G columns were equilibrated with binding buffer (Immunopure IgG, Pierce , USA after which a 1 1 mixture of sera and binding buffer was allowed to flow through under gravity, washed and eluted with elution buffer (Immunopure IgG, Pierce , USA.

Finally, 400 μl of Annexin V binding buffer was added.

400 µl of binding buffer was added before FACS analysis.

Then, 400  μl binding buffer was added.

An additional 300 μl binding buffer was then added.