Sentence examples for binding buffer were pre-incubated from inspiring English sources

Pure recombinant NEDD4L or NEDD4LΔWW proteins (0.5 μM in 500 μl binding buffer) were pre-incubated with washed resins for 1 hr, and then incubated for 1 hr with GFP- or AMOT-bound resins.

To avoid non-specific binding of BNIP3, slides were pre-incubated with 10% normal swine serum in PBS.

To block RAGE binding to ligands, cells were pre-incubated with a neutralizing anti-RAGE antibody and then incubated with 20 mM Rib.

Briefly, 20 µg nuclear proteins were pre-incubated with binding buffer for 10 min at 4°C and then incubated with biotin-labeled AP1 probe for another 20 min at room temperature.

Protein extracts (10 μg) in binding buffer supplemented with 100 μ m guanosine diphosphate (GDP) were pre-incubated for 30 min at 30 °C.

Egg extracts were pre-incubated with either buffer, recombinant cyclin B1-cdk1wt or cyclin B1-cdk1AF for 20 min at 30°C before CaCl2 addition.

10-µg aliquots of cell nuclear extracts were pre-incubated with 1 µg of poly dI-dc) in binding buffer (10 mM Tris [poly dI-dc0 mM NaCl, 20% glycerol, 1 mM dinhiothreitol [DTT], 0.5 mM EDTA) for 10 min at room temperature.

Nuclear extracts (5 10 μg) were pre-incubated on ice for 30 min in a binding buffer containing 10 mM Tris/HCl (pH 7.5), 50 mM NaCl, 0.5 mM EDTA, 5% glycerol and 1μg of poly dI-dC · dI-dC).

Sections were pre-incubated in an acidic buffer (pH = 4.40) to differentially inhibit myosin ATPases within the different fiber types.

For pH stability, the purified xylanases were pre-incubated in the different pH buffers for 1 h at 4 °C followed by activity determination at the optimal conditions.

MDA-MB-231 tumour sections (10 μm) were pre-incubated in 100 mM Tris-HCl buffer (pH 7.4), 5 mM CaCl2, 1 mM dithiothreitol (DTT) rt (20 min).