Sentence examples for binding buffer were analyzed from inspiring English sources

Cells diluted in binding buffer were analyzed by FACScan (BD Biosciences) and quantified by CellQuest software (BD Biosciences).

Cells resuspended in 500 μl annexin V binding buffer were analyzed by flow cytometry using a FACSVantage SE system (BD Biosciences) and analyzed using the FlowJo software.

TE buffer was analyzed as blank.

After addition of 200  μl Annexin V binding buffer, cells were analyzed with the FACSCanto II flow cytometer (BD Biosciences).

After adding 400 μl of 1× annexin V-FITC binding buffer, cells were analyzed using a flow cytometer (FACS Calibur; Becton Dickinson, Franklin Lakes, NJ).

Then 400 µl of 1× binding buffer were added to each sample which was analyzed by flow cytometry to quantify necrotic and apoptotic cells.

Finally, 400 μl of 1× binding buffer were added to each tube and the samples were analyzed by flow cytometry within 1 hour.

Before flow cytometric analysis, 400 μl of 1× binding buffer were added to the cells and the extent of apoptosis was determined by analyzing 15,000 ungated cells using a FACScan flow cytometer and Cell Quest software (FACSCalibur; Becton-Dickinson, San Jose, CA).

After incubation at room temperature for 15 min, 400 µL of binding buffer was added, and cells were analyzed by flow cytometry.

After 15 min incubation at room temperature, 400 ml of binding buffer was added, and cells were analyzed by flow cytometry using a FACSCalibur flow cytometer (Becton Dickinson, Franklin Lakes, NJ, USA).

After incubation, 400 μL of binding buffer was added and cells were analyzed in a FACScalibur (BD) using CellQuest software for determining the percentage of apoptotic cells.