Sentence examples for binding buffer were added from inspiring English sources

Then, 5 μg of cell membrane in 80 μL of binding buffer were added per well and incubated for 60 min at RT.

Before flow cytometric analysis, 400 μl of 1× binding buffer were added to the cells and the extent of apoptosis was determined by analyzing 15,000 ungated cells using a FACScan flow cytometer and Cell Quest software (FACSCalibur; Becton-Dickinson, San Jose, CA).

Then 400 µl of 1× binding buffer were added to each sample which was analyzed by flow cytometry to quantify necrotic and apoptotic cells.

After 30 min shaking at 4°C, viruses at a multiplicity of infection (MOI) of 500 VP/cell in 50 µl binding buffer were added into each tube, and they were shaken at 4°C for 1.5 hours.

Subsequently, another 400 μl of aliquot of binding buffer were added.

In all, 450  μl of binding buffer were added to terminate the reaction.

400 μL of 1X binding buffer was added to stop the staining reaction.

Finally, 400 μl of Annexin V binding buffer was added.

Before the detection, 200 μL of diluted binding buffer was added.

After mixing, 400 μl of binding buffer was added to each tube and apoptosis was analyzed using flow cytometer (Beckman Coulter, Inc., Fullerton, CA, USA).

Finally, 400 μL of the 1 × binding buffer was added to each tube, and analyzed by flow cytometry in 1 h.