Sentence examples for binding buffer was used from inspiring English sources

The EMSA binding buffer was used to prepare the working dilution.

Subsequently, binding buffer was used to wash the column, followed by elution buffer (20 mmol/L Tris HCl pH 7.9, 500 mmol/L imidazole, 0.5 mol/L NaCl).

After that, 15 ml of binding buffer was used to wash the column and then the r AciHBGase II-(His 6 protein was eluted with 5 ml of elution buffer (binding buffer except with 150 mM imidazole).

Annexin V Binding buffer was used to resuspend the pellet and Alexa Flour 488 annexin V and propidium iodide solution was added to the cells and incubated at room temperature for 15 minutes.

Binding buffer was used to serially dilute JAZ solutions for equilibration reactions so that each reaction had half of the protein concentration of the preceding sample.

After tagmentation, a Purelink PCR Purification Kit with HC Binding Buffer was used for purification and eluted with 30μL of EB or H2O.

Untreated cells re-suspended in DH20 for 15 minutes prior to adding binding buffer were used as a positive control for dead cells.

Ultra-15 Centrifugal Filters [30,000 MWCO; Millipore, Billerica, MA] equilibrated with binding buffer were used to reduce the volume to ~2 ml.

For FCM, cells (5 × 10 cells/ml) suspended in 500 μl binding buffer were used for DNA stain with 5 μl Annexin V-FITL and propidium iodine (PI, Sigma); then, DNA value, cell cycle and apoptotic rate of each group were determined by a cell apoptotic detection kit (BioDev, China) and a fluorescent activated cell sorter (420 type FCM, Becton-Dickinson, USA) as described previously [ 9, 12, 23].

Blocking buffer was used to avoid non specific binding.

RIPA buffer was used to reduce non-specific binding of actin to sepharose beads.