Sentence examples for binding buffer was transferred from inspiring English sources

A 100 μL of the suspended cells in binding buffer was transferred to a 5-mL culture tube, and 5 μL of Annexin V-FITC and 10 μL of PI were added.

The resuspended cells in 100 μL of 1 × binding buffer were transferred to a 1.5 mL culture tube, and 5 μL of FITC Annexin V and PI were added to each tube.

After staining, 350 μl of Annexin V binding buffer was added and all of the samples were transferred to Falcon flow tubes for analysis using FACSCanto Flow Cytometer (BD Bioscience, USA).

Finally, 400 μl of Annexin V binding buffer was added.

Before the detection, 200 μL of diluted binding buffer was added.

400 µl of binding buffer was added before FACS analysis.

Then, 400  μl binding buffer was added.

An additional 300 μl binding buffer was then added.

Four hundred microliters of binding buffer was added to each tube.

Afterwards, another 400 μL of binding buffer was added.

After incubation, the reaction mixture was made up to 100 µl with binding buffer before being transferred to a streptavidin-coated 96-well plate and incubated at 37°C for 1 h.