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Moreover, the presence of 0.15 0.2 M arginine in the binding buffer was shown to be more effective than salts like citrate and NaCl in reducing aggregate formation during elution [ 58].
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400 μL of 1X binding buffer was added to stop the staining reaction.
Briefly, Protein G columns were equilibrated with binding buffer (Immunopure IgG, Pierce , USA after which a 1 1 mixture of sera and binding buffer was allowed to flow through under gravity, washed and eluted with elution buffer (Immunopure IgG, Pierce , USA.
Finally, 400 μl of Annexin V binding buffer was added.
Before the detection, 200 μL of diluted binding buffer was added.
400 µl of binding buffer was added before FACS analysis.
Then, 400 μl binding buffer was added.
An additional 300 μl binding buffer was then added.
Four hundred microliters of binding buffer was added to each tube.
Afterwards, another 400 μL of binding buffer was added.
The binding buffer was Tris buffer (50 mM, pH 7.2) and elution buffer was Tris buffer with 1 mol/L of NaCl.
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