Sentence examples for binding buffer was saved from inspiring English sources

The 5th wash in 100 µl of binding buffer was saved as a wash control, and bound proteins were eluted with 100 µl of 3 M potassium thiocyanate.

400 μL of 1X binding buffer was added to stop the staining reaction.

Briefly, Protein G columns were equilibrated with binding buffer (Immunopure IgG, Pierce , USA after which a 1 1 mixture of sera and binding buffer was allowed to flow through under gravity, washed and eluted with elution buffer (Immunopure IgG, Pierce , USA.

Finally, 400 μl of Annexin V binding buffer was added.

400 µl of binding buffer was added before FACS analysis.

Then, 400  μl binding buffer was added.

Assembly of aptamer nanostructures in cell-binding buffer was performed as described above (using 1.5 µM C10.36 or Waz and 0.5 µM 3WJdB or AF488-anti-tail).

The sample volume was brought to 1 mL with RIPA buffer, and 100 μL was saved as an input control.

The labeled mitochondria were resuspended in fresh respiration buffer and the last wash supernatant was saved.

The supernatant was saved, and the pellet was resuspended in 100 μl of sucrose binding buffer.

A quantity of 100 μL of unsorted cells was saved and added directly to RLT buffer for the "unsorted" sample.