Sentence examples for binding buffer was replaced from inspiring English sources

After each time point, the plates were placed on ice, and the ligand binding buffer was replaced with ice-cold strip/stop solution.

Then, binding buffer was replaced and cells were stained with PI.

The blocking buffer was replaced with binding buffer containing labeled RNA and microarray slides incubated in the dark at 25°C for 1 hour with gentle agitation.

After the formation of GUVs, the outer buffer was replaced by a fresh buffer-1 containing 200 mM glucose.

The buffer was replaced after each measurement to avoid saturation of the oil in the incubating buffer.

For buffer desalting, the ribosome dilution buffer was replaced with PBS through ultrafiltration (Amicon Ultra 3000 Da MW; Merck, Darmstadt, Germany).

The blocking buffer was replaced with 100 µl of zipper-scFv G28-5 prorein or 293T cell lysates containing zipper-modified fiber proteins diluted in binding buffer (TBS with 0.05% Tween 20 and 0.05% casein).

The buffer was replaced with a fresh buffer containing 3.7 mM glucose for one hour before the buffer were collected (basal) and replaced with a buffer containing 16.7 mM glucose for an additional one hour (glucose stimulated).

Buffer was replaced every second day in all cases.

Subsequently, the fusion buffer was replaced by the imaging media.

On each occasion the collected buffer was replaced with fresh buffer to maintain constant volume.