Sentence examples for binding buffer was removed from inspiring English sources

Afterwards, the binding buffer was removed and the cells were washed twice with ice-cold phosphate-buffered saline (PBS; pH 7.4).

After incubation at ambient temperature for 1 h with gentle agitation, the binding buffer was removed using a multiscreen vacuum manifold (Millipore, Billerica, MA, USA).

Following RNA incubation binding buffer was removed and washing steps were implemented using 3 times exchange of 0.75 mL WB buffer: 40 mM Tris HCl (pH 8.0), 150 mM sodium chloride, 0.5 mM magnesium acetate, 10 μg/ml Yeast tRNA, 10 μg/mL heparin, 1 mM DTT, 0.01% Igipal 40, 5% Glycerol, 0.2 units/μl RNAseOUT for at least 5 minutes each.

Then, the Tris HCl buffer was removed.

Then buffer was removed and oocytes were fixed by addition of fixation buffer as described above.

Excess buffer was removed, leaving only interstitial buffer between packed eggs.

Then, the buffer was removed.

Staining buffer was removed and cells were washed with buffer.

The buffer was removed after vortexing.

The digest buffer was removed and saved.

Pellets were washed with PBS, and all buffer was removed.