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For TKu, the binding buffer was modified to contain 300 mM NaCl, keeping the concentration of other components the same.
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Staining recipes and gel buffers are modified from those of Shaw and Prasad [34].
Finally, 400 μl of Annexin V binding buffer was added.
Before the detection, 200 μL of diluted binding buffer was added.
400 µl of binding buffer was added before FACS analysis.
Then, 400 μl binding buffer was added.
An additional 300 μl binding buffer was then added.
Four hundred microliters of binding buffer was added to each tube.
Afterwards, another 400 μL of binding buffer was added.
The binding buffer was Tris buffer (50 mM, pH 7.2) and elution buffer was Tris buffer with 1 mol/L of NaCl.
The protein extraction protocol was modified by eliminating β-mercaptoethanol and polyvinylpolypyrrolidone from the extraction buffer.
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