Sentence examples for binding buffer was maintained from inspiring English sources

The final DMSO composition in the binding buffer was maintained at 2% v/v in the selectivity assays.

Before the binding assays, all reagents and buffers were maintained at room temperature.

400 μL of 1X binding buffer was added to stop the staining reaction.

Briefly, Protein G columns were equilibrated with binding buffer (Immunopure IgG, Pierce , USA after which a 1 1 mixture of sera and binding buffer was allowed to flow through under gravity, washed and eluted with elution buffer (Immunopure IgG, Pierce , USA.

Finally, 400 μl of Annexin V binding buffer was added.

Before the detection, 200 μL of diluted binding buffer was added.

400 µl of binding buffer was added before FACS analysis.

Then, 400  μl binding buffer was added.

An additional 300 μl binding buffer was then added.

Four hundred microliters of binding buffer was added to each tube.

Afterwards, another 400 μL of binding buffer was added.