Sentence examples similar to binding buffer was introduced from inspiring English sources

Buffer was introduced after agonist activation to deactivate the receptor.

An additional homogenization step after incubation in lysis buffer was introduced and pre-warmed buffers (protease buffer, lysis buffer and washing buffer) further enhanced the solubilization process.

400 μL of 1X binding buffer was added to stop the staining reaction.

Briefly, Protein G columns were equilibrated with binding buffer (Immunopure IgG, Pierce , USA after which a 1 1 mixture of sera and binding buffer was allowed to flow through under gravity, washed and eluted with elution buffer (Immunopure IgG, Pierce , USA.

Finally, 400 μl of Annexin V binding buffer was added.

Before the detection, 200 μL of diluted binding buffer was added.

400 µl of binding buffer was added before FACS analysis.

Then, 400  μl binding buffer was added.

An additional 300 μl binding buffer was then added.

Four hundred microliters of binding buffer was added to each tube.

Afterwards, another 400 μL of binding buffer was added.