Sentence examples for binding buffer was incubated from inspiring English sources
Pure recombinant HIV-1 GagΔp6 (1 μM) in 500 μl binding buffer was incubated with the washed resins for 1 hr.
Biotinylated peptide, 50 μg, in binding buffer was incubated with 250 μl of streptavidin-coated agarose slurry (PIERCE, ProFoundTM Pull-Down kit) for 1 h at 4°C rotating in a 500 μl reaction, blocked with free biotin for 5 min, washed with HS buffer and blocked with 1 mg/ml BSA overnight at 4°C.
To examine the effects of amino acids on PLG binding to the pathogen surface, 5×107 Sterne spores in binding buffer were incubated with 2 µg of PLG and 50 mM amino acids for 1 h on a rocker platform.
2×105 cells in 100 µl of binding buffer were incubated with 2 µl of annexin V-PE and 2 µl of 7-amino-actinomycin D for 15 min at 25 °C in the dark.
Then, 100 μl binding buffer containing 10 kBq of In-DOTA-E-[c RGDfK ]2 and approprIn-DOTA-E-[c RGDfK ]20−6–8 × 10−11 M) of Ga(In-DOTA-E-[c RGDfK ]2lled In-DOTA-E-[c RGDfK ]2-E-[c(RGDfK)]2 and DOTappropriatefK)]2}2 in bindilutionser were incubated in the wells at 37°C for 1 h.
In general, DNA-binding experiments were carried out in the following manner: 5 μL NF-D1 (consisting of NF-01F and NF-14D) and 5 μL protein in binding buffer were incubated in a 96-well (half-area) microplate for 30 min at room temperature.
In saturation binding experiments, membranes in HEM buffer were incubated with various concentrations of [3H]Prazosin in a total volume of 200 µL.
Pure recombinant NEDD4L or NEDD4LΔWW proteins (0.5 μM in 500 μl binding buffer) were pre-incubated with washed resins for 1 hr, and then incubated for 1 hr with GFP- or AMOT-bound resins.
After washing three times in TSM washing buffer, 100 µl of anti-His tag-HRP antibody diluted 1∶3 000 in binding buffer was added to each well and incubated for 2h at room temperature.
