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The binding buffer was allowed to flow over the sensor chip for 15 min at a rate of 30 μL/min to allow for the dissociation of bound MAb/scFv from the antigen on the chip.
The binding buffer was allowed to drain to the top of the column bed before loading the column with the prepared extract.
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Buffer was allowed to reach steady state.
The falcon tubes were vortexed and allowed to sit at room temperature for 10 min before another 1 mL aliquot of 1× binding buffer was added.
Finally, 400 μl of Annexin V binding buffer was added.
Before the detection, 200 μL of diluted binding buffer was added.
400 µl of binding buffer was added before FACS analysis.
Then, 400 μl binding buffer was added.
An additional 300 μl binding buffer was then added.
Four hundred microliters of binding buffer was added to each tube.
Afterwards, another 400 μL of binding buffer was added.
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