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The pH of the buffer was adjusted with orthophosphoric acid.
The pH of the buffer was adjusted to 7.4.
The pH of the buffer was adjusted to 7.4 using a precalibrated pH meter.
The pHs of these buffer was adjusted to 5 and 6, respectively.
Buffer was adjusted to desired pH using HCl and NaOH.
The pH of the elution buffer was adjusted to 3.9 using 0.3 M acetic acid.
Buffer was adjusted to pH 7.4 or pH 6.0 using HCl and NaOH.
The pH of all buffers was adjusted at 25 °C.
Buffers and NaCl solution were also made up in MilliQ water and buffers were adjusted to 7.4 with aq.
Finally, 400 μl of Annexin V binding buffer was added.
400 µl of binding buffer was added before FACS analysis.
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