Sentence examples for binding buffer was added from inspiring English sources

400 μL of 1X binding buffer was added to stop the staining reaction.

Finally, 400 μl of Annexin V binding buffer was added.

Before the detection, 200 μL of diluted binding buffer was added.

Finally, 400 μL of the 1 × binding buffer was added to each tube, and analyzed by flow cytometry in 1 h.

After mixing, 400 μl of binding buffer was added to each tube and apoptosis was analyzed using flow cytometer (Beckman Coulter, Inc., Fullerton, CA, USA).

After incubation, 400 μL 1× binding buffer was added to each tube, and the samples were analyzed by flow cytometry (Beckman-Coulter) within 1 h.

At the end of incubation, 400 μL of binding buffer was added, and the cells were analyzed immediately by flow cytometry.

The following day, 180 µl of fresh serum-free medium containing Waz 3WJdB Cy3 (1.5 µM Waz and 0.5 µM 3WJdB Cy3), and a mix of tRNA + ssDNA (0.5 mg/ml each) in cell-binding buffer was added to cells.

After incubation at room temperature for 15 min, annexin-binding buffer was added and the stained cells were analyzed by FACS.

After the incubation period, 400 µL 1× Annexin-binding buffer was added, mixed gently and the samples were placed on ice.

Then, 400 μL of 1× Annexin-binding buffer was added and mixed gently.