Sentence examples for binding buffer using the from inspiring English sources

Incubation was stopped by rapid filtration and rinsing with ice-cold binding buffer, using the Brandel harvester (Gaithersburg, MD) and Whatman GFB filter paper (Brandel, Gaithersburg MD).

Unbound sample was washed from the column with binding buffer using an ÄKTA explorer (GE Healthcare).

The binding reactions (30 min at room temperature in 20 μl) were carried out in the presence of 2.5 ng of poly dI-dC) in 1× binding buffer using 100 poly dI-dCotin-labelled duplex probe and, where indicated, a 20-fold molar excess of unlabelled probe.

Samples were exchanged into ITC binding buffer using NAP-25 desalting columns.

To remove unbound DNA, each sample was then washed three times in Ang2 binding buffer using a magnetic particle concentrator.

The manufacturer's instructions were followed with the exception that an equal volume of 70% ethanol was added to the binding buffer used in step one of the kit's instructions.

Since their storage occurs in 20% ethanol, first of all the magnetic beads were washed for four times with 0.5 mL wash- and binding buffer by using a magnetic device for removal of the supernatant.

Endogenous peroxidase activity was blocked using 0.3%H2O2/PBSS for 10 min at room temperature and non-specific binding sites using the blocking buffer of the VECTASTAIN Elite ABC Kit (VECTOR Laboratories, Peterborough, UK) for 45 min.

Endogenous peroxidase activity was blocked using 0.3%H2O2/phosphate-bufferedH2O2/phosphate-buffered at room temperature and nonspecific binding sites using the blocking buffer of the VECTASTAIN Elite ABC Kit (VECTOR Laboratories, Peterborough, UK) for 45 minutes at room temperature.

After this incubation period, the cells were washed twice with ice cold binding buffer, harvested using 5 mM EDTA, washed again and resuspended in ice cold binding buffer containing 4 μg/mL Alexa488 quenching polyclonal antibody.

The EMSA binding buffer was used to prepare the working dilution.