Sentence examples for binding buffer using a from inspiring English sources

To remove unbound DNA, each sample was then washed three times in Ang2 binding buffer using a magnetic particle concentrator.

Unbound sample was washed from the column with binding buffer using an ÄKTA explorer (GE Healthcare).

The binding reactions (30 min at room temperature in 20 μl) were carried out in the presence of 2.5 ng of poly dI-dC) in 1× binding buffer using 100 poly dI-dCotin-labelled duplex probe and, where indicated, a 20-fold molar excess of unlabelled probe.

Incubation was stopped by rapid filtration and rinsing with ice-cold binding buffer, using the Brandel harvester (Gaithersburg, MD) and Whatman GFB filter paper (Brandel, Gaithersburg MD).

Samples were exchanged into ITC binding buffer using NAP-25 desalting columns.

The manufacturer's instructions were followed with the exception that an equal volume of 70% ethanol was added to the binding buffer used in step one of the kit's instructions.

Since their storage occurs in 20% ethanol, first of all the magnetic beads were washed for four times with 0.5 mL wash- and binding buffer by using a magnetic device for removal of the supernatant.

After incubation at ambient temperature for 1 h with gentle agitation, the binding buffer was removed using a multiscreen vacuum manifold (Millipore, Billerica, MA, USA).

Cells were washed in binding buffer and analyzed using a MACSQuant flow cytometer with MACSQuantify software (Miltenyi Biotec).

After 15 min of incubation in the dark at room temperature, the cell suspensions were adjusted to 500  μl with 1X binding buffer and counted using a FacScan analyzer (argon laser; Becton Dickinson, Franklin Lane, NJ, USA).

Untreated cells re-suspended in DH20 for 15 minutes prior to adding binding buffer were used as a positive control for dead cells.