Sentence examples for binding buffer supplied by from inspiring English sources

Single-cell suspensions were then prepared by trypsinization and resuspended in 500 μl of binding buffer supplied by the manufacturer.

After 24 h incubation, cells were washed with 1X-PBS and re-suspended in 100μl binding buffer (supplied by the vendor).

After incubation, cells were washed with Dulbecco's phosphate buffered saline (DPBS) (Sigma, St . Louis MO, USA) and resuspended in 100 μl binding buffer (supplied by the vendor).

Then three more washing steps were carried out with 5 mL wash- and binding buffer supplied with additives for the particular enzyme.

Next, cells were resuspended in 1x annexin V binding buffer supplied with the Apoptosis and Necrosis Quantification kit.

Briefly, live cells were incubated in binding buffer supplied with the kit, along with Annexin V-Cy3 (1 100), propridium iodide (TOPRO-3, 1 1000) and Hoechst 33342 (1:2000, Molecular Probes).

All ctDNA markers were amplified with NEB Taq using the buffer supplied by the manufacturer (NEB co., Japan).

LDH release induced by the lysis buffer supplied by the manufacturer was set at 100%.

Liver tissues were homogenized in lysis buffer supplied by the vendor.

The cells were lysed by incubating the slides in lysis buffer (supplied by the kit; Trevigen) for 2 h.

RNAs were then phosphorylated using T4 PNK NEBB) in the buffer supplied by the manufacturer for 1.5 hr at 37°C.